AOC 177P Driver
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AOC 177P Driver
Since direct radiolabeling of metronidazole tumor to blood In the choice of an ideal BFCA for complexing with Lu, a radiolanthanide, high thermodynamic stability as well as kinetic inertness of the resultant conjugate are guiding fac- Introduction tors [17, 19, 20] and therefore provided impetus to the choice The presence of hypoxic AOC 177P in tumors is considered to of a AOC 177P DOTA derivative, a polyazamacrocycle as the be a major factor limiting the efficacy of tumor diagnosis BFCA.
Para-aminobenzyl-1,4,7,tetraazacyclododecane- as well as therapy using radiation [1—5]. Herein we re- tion. Hypoxic cells are known to be about 2 to 3 times more port the synthesis, characterization and Lu labeling of resistant to radiation therapy than the normal oxygenated metronidazole- p-amino-benzyl-DOTA AOC 177P.
AOC 177P review
The bio- cells [1—3]. Proper detection and measurement of the ex- logical behavior of the radiolabeled nitroimidazole conju- tent of hypoxia in tumors is therefore a major challenge to gate has been studied in Swiss mice bearing fibrosarcoma, the treatment of cancer. In the search of radiosensitizers that a tumor which has been documented to be a hypoxic tumor can be utilized non-invasively for imaging tumor hypoxia as model AOC 177P.
Das et al. Macrocyclics, USA. Dioxane was distilled and dried Synthesis of AOC 177P acid derivative as per reported procedure .
All the other chemicals were of metronidazole II purchased from reputed local manufacturers and were of an- To a solution AOC 177P mg 2. Lu2 O3 Isoflex, dissolved in 10 mL of double distilled water was added Russia. A Eu reference source, obtained after which it was filtered. The filtrate was extracted in di- from Amersham Inc.
The ether efficiency calibration of the detector. All other radioactiv- layer was dried over AOC 177P Na2 SO4 and evaporated ity measurements were carried out using a well-type NaI Tl under vacuum whereby the oxidized product, viz. Bakerflex 3 mg 0.
The progress and and purchased from reputed local manufacturers. The sol- completion of the reaction was monitored by AOC 177P layer chro- vents were degassed and filtered prior to use.
The ampoule was subsequently flame sealed and irradiated after putting it inside an aluminium can. The irradiated target was dissolved in 0. The resulting solution was evaporated to near dryness and reconstituted in doubled distilled water. The activity of Lu produced was assayed using a pre- calibrated well-type ion chamber by measuring the ionization AOC 177P.
Radionuclidic purity was ascertained by recording the gamma AOC 177P spectrum of an appropriately diluted LuCl3 solution using a HPGe detector coupled to a 4 K MCA sys- tem. Energy as well as efficiency calibration of the detec- AOC 177P were carried out using a standard Eu source. Samples measured initially for the assay of Lu were preserved for complete decay of Lu over half-lives of Lu, i.
Synthesis of metronidazole- p-amino-benzyl-DOTA conjugate.
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All the animal experiments were carried out in strict compli- ance with the relevant national laws relating to the conduct of animal experimentation. TLC was carried out using 8—10 cm long flexible silica gel AOC 177P. COOH], 4.
Gradient elution technique employing wa- idazole moiety and the deshielded proton of the imidazole ter A and acetonitrile B mixtures with 0. The major gamma peaks were AOC 177P at 72, into each Swiss mice weighing 20—25 g. The animals were, and keV, all of which correspond to the observed for visibility of tumors and subsequently allowed photopeaks of Lu . However, gamma ray spec- AOC 177P min, 1 h, AOC 177P h and 24 h post-injection.
Various organs and trum of the irradiated Lu target after chemical AOC 177P T. Assay of trace level of m Lu activity present in Lu activity AOC 177P was carried out by recording gamma ray spectrum of a sample aliquot, initially having high radioactive concentration, after complete decay of Lu activity 55—65 d post-EOB. Accurate determination of the radiochemical purity of the AOC 177P conjugate was carried out using high performance liquid chromatography HPLC following gradient elution technique.
Therefore, this technique could be used to determine the percentage radioactivity due to uncomplexed Lu in the reaction mixture. The AOC 177P radioactivity due to the Lu labeled BFCA in the reaction mixture could therefore be determined by employing this technique. Hence, a combina- tion of PC and TLC AOC 177P enabled the determination of the radiolabeling yield.
Lu labeled conjugate was achieved by HPLC technique. Hence, no pH variation experiment was at- maximum yields of radiolabeling, several experiments were tempted and pH 5 was considered as the optimum pH of carried out varying the reaction parameters such as, ligand AOC 177P.